Pharm Sci. 2019;25(2): 118-123.
doi: 10.15171/PS.2019.18

Scopus ID: 85069209477
  Abstract View: 321
  PDF Download: 195

Research Article

MCF-7 and its Multidrug Resistant Variant MCF-7/ADR Overcome TNF Cytotoxicity through Prevention of Reactive Oxygen Species Accumulation

Morteza Ghandadi 1 ORCID logo, Javad Behravan 2,3, Samira Biabani 2, Sara Abbaspor 2, Fatemeh Mosaffa 2,3 * ORCID logo

1 Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
2 Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
3 Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.


Background: Signal transduction of numerous cytokines and growth factors are mediated by reactive oxygen species (ROS). Tumor necrosis factor-α (TNF-α) have stimulated accumulation of ROS in various in vitro studies. MCF-7 and its Adriamycin resistant variant MCF-7/ADR are resistant against TNF-α cytotoxicity. Role of ROS in the resistance of MCF-7 and MCF-7/ADR cells was investigated. Methods: ROS accumulation and viability in MCF-7 and MCF-7/ADR after TNF-α exposure was evaluated using 2',7'-dichlorofluorescein diacetate (DCFH-DA) as a fluorescent probe and 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide (MTT) cytotoxicity assay respectively. Results: ROS level did not change significantly after TNF-α exposure. Induction of ROS accumulation along with TNF-α treatment sensitized these cells to TNF-α toxicity. Conclusion: It can be concluded that lack of ROS accumulation following TNF-α exposure is involved at least by part in the resistance of MCF-7 and its drug resistant derivative MCF-7/ADR cells to TNF-α cytotoxicity.
Keywords: Breast cancer, MCF-7, Multidrug resistance;, ROS, TNF-α
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Submitted: 28 Jan 2019
Revision: 18 Mar 2019
Accepted: 18 Mar 2019
ePublished: 30 Jun 2019
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