Khashayar Namvar
1, Ameneh Mohammadi
2, Esmail Ataei Salehi
2, Peyman Feyzi
2*1 Department of Food Science and Technology, Islamic Azad University, Quchan Branch, Iran.
2 Natural Products & Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.
Abstract
Background: The genus of
Stachys with 300 species belongs to Lamiaceae family and this genus
has 34 species in Iran. Stachys turcomanica Trautv is native to Iran and
it used for treatment of infectious, rheumatic and inflammatory diseases in
traditional medicine. The effect of solvent (methanol in water: 0, 20, 50, 80
and 100 %) on the extraction of antioxidant compounds from S. turcomanica were
investigated using various in vitro assays.
Methods: The antioxidant
activities of extracts were studied via three methods: FRAP (ferric reducing
antioxidant power), DPPH (2, 2-diphenyl-1-picrylhydrazyl) and beta-carotene
linoleic acid bleaching.
Results: The results of
present work revealed that the solvent combinations have effects on the extraction of phenolic
compounds and antioxidant properties. The relative
methanol/water ratio of 80:20 v/v was effective solvent in the extracting of
phenolic compounds. Also, there was a good correlation between antioxidant activity
and total phenolic content S. turcomanica
extracts.
Conclusion: The results demonstrated
methanol/water (80:20) ratio was the best solvent to release of many secondary
metabolites from S. turcomanica
for
future studies, which could provide natural sources of antioxidant compounds.