Logo-ps
Pharm Sci. 2021;27(2): 262-267.
doi: 10.34172/PS.2020.52
  Abstract View: 275
  PDF Download: 101

Research Article

Anti-Cancer Effects of Probiotic Lactobacillus acidophilus for Colorectal Cancer Cell Line Caco-2 through Apoptosis Induction

Alireza Isazadeh 1,2 ORCID logo, Saba Hajazimian 1, Behrouz Shadman 3, Sahar Safaei 1, Ahmad Babazadeh Bedoustani 4, Reza Chavoshi 2, Dariush Shanehbandi 1, Mohammadreza Mashayekhi 2, Mohammadreza Nahaei 5* ORCID logo, Behzad Baradaran 1,6* ORCID logo

1 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Department of Genetics, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
3 Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey.
4 Biotechnology Research Center, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
5 Department of Microbiology and Laboratory Sciences, School of Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
6 Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

Background: Colorectal cancer is one of the most common cancers worldwide. Probiotics are useful and non-pathogenic microorganisms in the gastrointestinal tract, which can show anticancer activity through the induction of apoptosis. This study aimed to evaluate the antiproliferative effects of Lactobacillus acidophilus probiotic on the Caco-2 colorectal cancer cell line.

Methods: The supernatant (secreted metabolites) and bacterial extract of L. acidophilus probiotics were prepared and used as an anti-proliferative agent on the colorectal cancer cell line, Caco-2 in vitro. The effects of supernatant and extract of L. acidophilus were evaluated on the viability and proliferation of cancer cells using MTT assay. Moreover, morphological alterations of cancer cells treated with supernatant and extract of L. acidophilus were evaluated by an inverted phase contrast microscope. The mRNA expression levels of apoptosis-related genes (SURVIVIN and SMAC) in treated cancer cells and untreated controls were evaluated using the Real-Time PCR method.

Results: The results showed that the supernatant and extract of L. acidophilus inhibited the viability and proliferation of cancer cells in a dose and time-dependent manner. Moreover, various morphological alterations were observed in the treated cancer cells, which are indicators of apoptosis induction. The mRNA expression of SURVIVIN and SMAC genes were significantly up-regulated and downregulated in the treated cancer cells, respectively.

Conclusion: The results of the present study suggested that the supernatant and extract of L.acidophilus could inhibit the viability and proliferation of colorectal cancer cell line, Caco-2through induction of apoptosis, increase the survival rate of colon cancer patients.

Keywords: Apoptosis, Colorectal cancer, Lactobacillus, Probiotics
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 276

Your browser does not support the canvas element.


PDF Download: 101

Your browser does not support the canvas element.

Submitted: 23 Mar 2020
Revision: 24 Jun 2020
Accepted: 25 Jun 2020
ePublished: 02 Oct 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)