Sajedeh Bahrani
1,2, Babak Ghanbarzadeh
2, Mahmoud Sowti Khiabani
2, Saeed Ghanbarzadeh
3,4, Hamed Hamishehkar
5*1 Biotechnology Research Center, and Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
3 Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
4 Zanjan Pharmaceutical Nanotechnology Research Center and Department of Pharmaceutics, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
5 Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
Background: The formation of electrostatic complexes
between two types of biopolymers, sodium Caseinate (a derivative from most
abundant milk protein) and Pectin (a natural hetro polysaccharide), was studied
as a function of biopolymers concentrations and pH of solutions (3.9- 4.3).
Method: The
size and morphology of the resulted complexes were investigated by using of
laser light scattering and transmission electron microscopy, respectively.
Response surface methodology (A three-factor, three levels Box-Behnken design)
was used for the optimization procedure with pH, pectin and sodium Caseinate
concentrations as independent variables. Particle size and polydispersity index
of nanocomplexes were considered as dependent variables.
Results: Negatively
charged nanocomplexes were produced below the isoelectric point of protein
(5.4), at pH 4.1 with a suitable colloidal stability and average particle size
of about 100 nm. It was found that the particle size of nanocomplexes could be
controlled by changing in variables.
Conclusion: In
conclusion response surface methodology are simple, rapid and beneficial
approach for preparation, optimization and investigation of the effect of
independent variables on the properties of products.