Ali Ghashghaii
1*, Mohammad Hashemnia
2, Zahra Nikousefat
1, Mohammad Mahdi Zangeneh
2, Akram Zangeneh
21 Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
2 Department of Pathobiology, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
Abstract
Background: Scrophularia
striata is a well-known plant in Iranian traditional medicine and its
anti-oxidative and anti-inflammatory properties make it a logical adjuvant to
improve wound healing. This study was designed to evaluate the wound healing
potential of S. striata on cutaneous wounds in rat.
Methods: A full-thickness excisional wounds was induced on
the back of 75 Sprague-Dawley rats. The animals were randomly allocated into
five groups, treated with 1ml basal cream, 1ml tetracycline (3%), 1ml S.
striata 5%, 1ml S. striata 10% and untreated (control). Five animals of each
group were euthanized at each of 10, 20 and 30-days post-injury (DPI) and
wounds were assessed through gross and histopathological analyses.
Results: Treated rats with S. striata showed a significant
decrease in the wound area during the experiment compared to other groups.
Additionally, treatment with S. striata decresed the number of lymphocytes and
enhanced the number of fibroblasts at the earlier stages and increased number
of fibrocytes at the later stages of wound healing. Other parameters such as
alignment of the healing tissue, re-epithelilization and epithelial formation,
enhanced maturity of the collagen fibers and fibroblasts and large
capillary-sized blood vessels showed significant changes when compared to
control. The best wound healing activity was observed with the high dose of S.
striata.
Conclusion: The present study showed that
application of S. striata extract on wounds induces considerable wound
contraction and accelerates healing and it may be suggested for treating
different types of wounds in animal and human beings.