Pharm Sci. 2017;23(3): 198-206.
doi: 10.15171/PS.2017.30

Scopus ID: 85032008645
  Abstract View: 2540
  PDF Download: 2246

Research Article

Antibacterial Activity of Copper Oxide (CuO) Nanoparticles Biosynthesized by Bacillus sp. FU4: Optimization of Experiment Design

Mojtaba Taran 1, Maryam Rad 1, Mehran Alavi 1*

1 Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.


Background: There are several methods for synthesis of metallic nanoparticles (NPs) including chemical, physical and biological process. In this study, Bacillus sp. FU4 was used as biological source for biosynthesis of CuO NPs. Methods: CuO NPs have been prepared by copper sulfate (CuSO4). CuO NPs were formed after oxidation of Cu NPs. Design and analysis of Taguchi experiments (an orthogonal assay and analysis of variance (ANOVA)) carried out by the Qualitek-4 software. Average effect of CuSO4 concentration (0.1, 0.01 and 0.001 M), incubation and culturing time (48, 72, 96 hours) as three controllable factors with three levels were evaluated in CuO NPs biosynthesis. Characterization of CuO NPs was determined by UV-Vis spectroscopy, X-ray diffraction (XRD), Fourier transform infra-red (FT-IR) spectroscopy and scanning electron microscopy (SEM). Also, the antimicrobial properties of CuO NPs were investigated using Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 43300 as multidrug resistant (MDR) bacteria. Results: Results: It was evaluated that, NPs size distributions were in the range of 2-41 nm with spherical shapes. The anti-bacterial activities of CuO NPs were measured based on diameter of inhibition zone in disk diffusion tests of NPs dispersed in batch cultures. Two levels of CuSO4 concentrations (0.1 and 0.01M) had antibacterial effect on E.coli (33±0.57 and 6 ±2mm). In the case of S. aureus, there was surprisingly no sign of growth. Conclusion: CuO NPs have antibacterial activity that can be benefit in medicinal aspect for fighting against prominent pathogen bacteria such as E.coli ATCC 25922 and S.aureus ATCC 43300.
Keywords: Green chemistry, Bacillus sp. FU4, Taguchi method, CuO nanoparticles, Escherichia coli ATCC 25922
First Name
Last Name
Email Address
Security code

Abstract View: 2541

Your browser does not support the canvas element.

PDF Download: 2246

Your browser does not support the canvas element.

Submitted: 14 Apr 2017
Accepted: 05 Jul 2017
ePublished: 30 Sep 2017
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)