Mojtaba Taran
1, Maryam Rad
1, Mehran Alavi
1*1 Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
Abstract
Background: There
are several methods for synthesis of metallic nanoparticles (NPs) including
chemical, physical and biological process. In this study, Bacillus sp. FU4 was
used as biological source for biosynthesis of CuO NPs.
Methods:
CuO NPs have been prepared by copper sulfate (CuSO4). CuO NPs were
formed after oxidation of Cu NPs. Design and analysis of Taguchi experiments
(an orthogonal assay and analysis of variance (ANOVA)) carried out by the
Qualitek-4 software. Average effect of CuSO4 concentration (0.1,
0.01 and 0.001 M), incubation and culturing time (48, 72, 96 hours) as three
controllable factors with three levels were evaluated in CuO NPs biosynthesis.
Characterization of CuO NPs was determined by UV-Vis spectroscopy, X-ray
diffraction (XRD), Fourier transform infra-red (FT-IR) spectroscopy and
scanning electron microscopy (SEM). Also, the antimicrobial properties of CuO
NPs were investigated using Escherichia coli ATCC 25922 and Staphylococcus
aureus ATCC 43300 as multidrug resistant (MDR) bacteria.
Results:
Results: It was evaluated that, NPs size distributions were in the range of
2-41 nm with spherical shapes. The anti-bacterial activities of CuO NPs were
measured based on diameter of inhibition zone in disk diffusion tests of NPs
dispersed in batch cultures. Two levels of CuSO4 concentrations (0.1
and 0.01M) had antibacterial effect on E.coli (33±0.57 and 6 ±2mm). In the case
of S. aureus, there was surprisingly no sign of growth.
Conclusion:
CuO NPs have antibacterial activity that can be
benefit in medicinal aspect for fighting against prominent pathogen bacteria
such as E.coli ATCC 25922 and S.aureus ATCC 43300.