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  Abstract View: 75

Research Article

Green Curmin reduces pro-inflammatory cytokines and fibroblast-associated colon cancer migration

Nasapon Povichit, Tharathip Muangthong ORCID logo, Parichaya Aimvijarn, Prasit Suwannalert* ORCID logo

Abstract

Introduction: Green Curmin is a soluble curcumin extract product made in Thailand and reported to reduce gastric inflammation. Inflammatory cytokines including IL-1α, IL-6 and TNF-β play a crucial role in cancer cell migration, which is a major process towards poor prognosis. Objective: To investigate the effects of Green Curmin on colon cancer cell growth and migration, which involve the pro-inflammatory cytokines. Method: Curcuma longa, L. were extracted and named as Green Curmin. The cytotoxicity of the Green Curmin-treated HCT116-colon cancer cells was obtained by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. The effect of the extract on cancer migration was investigated with wound healing assay. In addition, fibroblast-associated cancer migration was confirmed by using Transwell migration assay. Pro-inflammatory cytokines were also determined. Results: Green Curmin had cytotoxicity in the induced-cancer cell apoptosis at concentrations of 1 and 5 mg/ml. However, at concentrations of 0.05 and 0.1 mg/ml (non-toxicity dose), andreduced colon cancer migration and fibroblast associated-cancer migration. They also suppressed pro-inflammatory cytokines expression including IL-1α, IL-6 and TNF-β in colon cancer cells and IL-6 in fibroblast cells. Conclusion: Green Curmin suppressed pro-inflammatory cytokines and reduced fibroblast associated-cancer migration.


Keywords: Green Curmin, Inflammation, Colon cancer, Fibroblast, Migration
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Submitted: 03 Aug 2020
Revision: 10 Feb 2021
Accepted: 10 Feb 2021
ePublished: 15 Feb 2021
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