Logo-ps
Pharm Sci. 2024;30(4): 496-501.
doi: 10.34172/PS.2024.26
  Abstract View: 28
  PDF Download: 30

Research Article

Cytotoxic Flavonoids from Lannea egregia Engl. & K. Krause

Oluwatosin T. Ayodele 1,2,3 ORCID logo, Olaoluwa O. Olaoluwa 1, Saheed O. Benson 3 ORCID logo, Olapeju O. Aiyelaagbe 1 ORCID logo, Lutfun Nahar 4* ORCID logo, Amos A. Fatokun 3* ORCID logo, Satyajit D. Sarker 3 ORCID logo

1 Department of Chemistry, University of Ibadan, Ibadan, Oyo State, 200284, Nigeria.
2 Department of Chemistry, Chrisland University, Ajebo Road, Abeokuta, Ogun State, 110222, Nigeria.
3 Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, United Kingdom.
4 Laboratory of Growth Regulators, Palacký University and Institute of Experimental Botany, The Czech Academy of Sciences, Šlechtitelů 27, 78371 Olomouc, Czech Republic.
*Corresponding Authors: Lutfun Nahar, Email: nahar@ueb.cas.cz; Amos A. Fatokun, Email: A.A.Fatokun@ljmu.ac.uk

Abstract

Background: Lannea egregia Engl. & K. Krause (family: Anacardiaceae) is a well-known medicinal plant in Nigeria whose various parts have been shown to elicit several biological activities. This study specifically explored the leaf of L. egregia for potential cytotoxic compounds.

Methods: n-Hexane, dichloromethane and methanolic extracts of the leaf were prepared using the Soxhlet apparatus and concentrated using the rotary evaporator. Compounds were isolated by reversed-phase preparative high-performance liquid chromatography, and the structures were determined by spectroscopic means. The methanolic extract and the isolated compounds were screened for cytotoxicity against HeLa and MCF-7 cancer cell lines, using the MTT assay.

Results: Three flavonoids, myricetin (1), myricetin 3-O-α-L-rhamnoside (2) and quercetin 3-O-α-L-rhamnoside (3), were isolated from the methanolic extract of the leaf of L. egregia. The methanolic extract and compound 3 showed the most potent inhibition profiles against the cells, with IC50 values (Mean ± SEM) of 45.3 ± 1.5 µg/mL and 57.5 ± 0.4 µg/mL for the methanolic extract, and 36.5 ± 2.0 µM and 57.9 ± 10.1 µM for compound 3, against HeLa and MCF-7 cells, respectively. Conclusion:

Conclusion: This work shows that L. egregia leaf is moderately cytotoxic, and rich in flavonoids, and the cytotoxicity of the extract is, at least partially, due to the presence of cytotoxic flavonoids. This is the first report on characterized isolated compounds from the leaf of L. egregia and the occurrence of flavonols in the leaf.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 29

Your browser does not support the canvas element.


PDF Download: 30

Your browser does not support the canvas element.

Submitted: 13 Apr 2024
Accepted: 03 Aug 2024
ePublished: 07 Sep 2024
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)