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Pharm Sci. 2024;30(4): 423-431.
doi: 10.34172/PS.2024.12
  Abstract View: 63
  PDF Download: 28

Research Article

Electromembrane Extraction of Ofloxacin from Human Plasma and Its Quantification by Capillary Electrophoresis

Mahsa Javan 1 ORCID logo, Behrouz Seyfinejad 1,2, Elaheh Rahimpour 1,3, Mojtaba Varshochi 3, Abolghasem Jouyban 1,4* ORCID logo

1 Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
*Corresponding Author: Abolghasem Jouyban, Email: ajouyban@hotmail.com

Abstract

Background: Application of electromembrane extraction coupled with capillary electrophoresis was studied for ofloxacin extraction from plasma samples.

Methods: Ofloxacin migrated from acidic plasma samples through a thin layer of 1-octanol immobilized in the pores of a porous hollow fiber wall into a 10 µL acidic aqueous acceptor solution located inside the lumen of the fiber.

Results: Under optimum conditions influencing electromigration (i.e., 20 min of the operation time, stirring speed of 750 rpm, donor phase pH at 4.0, acceptor pH at 3.0, and applied voltage of 30 V across the supported liquid membrane), ofloxacin was extracted from plasma samples with an enrichment factor of 100-fold corresponding to extraction percent of 24%. The calibration curve showed acceptable linearity in the range of 0.2-7.0 µg.mL-1 (R=0.9993). The limits of detection and quantification of 0.05 and 0.2 µg.mL-1 were obtained, respectively. The inter- and intra-day precision and accuracy were obtained below 8.65%.

Conclusion: The validated method was successfully processed for the ofloxacin determination in the plasma samples of patients under ofloxacin therapy. There were no interfering peaks which indicates the great selectivity of the developed method.

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Submitted: 26 Feb 2024
Accepted: 10 Apr 2024
ePublished: 08 Jul 2024
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