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Pharm Sci. 2022;28(1): 145-155.
doi: 10.34172/PS.2021.54

Scopus ID: 85127181104
  Abstract View: 973
  PDF Download: 701

Research Article

Microencapsulation of Enriched Extracts of Two Satureja Species by Spray Drying, Evaluation of the Controlled Release Mechanism and Cytotoxicity

Faezeh Fathi 1 ORCID logo, Samad Nejad Ebrahimi 1* ORCID logo, David Pereira 2, Berta Estevinho 3, Fernando Rocha 3

1 Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, 1983969411 Tehran, Iran.
2 REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, no 228, 4050-313 Porto, Portugal.
3 LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
*Corresponding Author: Email: s_ebrahimi@sbu.ac.ir

Abstract

Background: Phenolic compounds are one of the main groups of secondary metabolites responsible for multiple biological and pharmacological properties that play a vital role in improving human health quality. Encapsulation by spray dryer creates protection toward the phenolic compounds as an efficient way for increasing product performance.

Method: The phenolic compounds of Satureja khuzistanica Jamzad (SKH) and S. rechingeri Jamzad (SRH) were enriched based on adsorbent resin column chromatography and the enrichment index was confirmed by HPLC-UV analysis. Gum Arabic, carboxylated chitosan, and pectin with the optimum percentage of 1% w/w used to encapsulate SKH and SRH by the spray drying technique.

Result: Encapsulation yield was 38.18 – 59.00 %, particle size ranged 2.278 - 4.689 µm, and release time was between 4.08 - 82.08 min. The gum Arabic-based capsules showed the fastest and pectin-based revealed the slowest release time. The best statistical model explained a release mechanism was Korsmeyer model. Anomalous transport was observed from all formulas except SKH-gum Arabic (case-I transport), SKH-pectin, and SRH-carboxylated-chitosan (super case-II transport). The cytotoxic activity of encapsulate SKH’s revealed reducing the viability of AGS evaluated by the MTT compared with SRH’s.

Conclusion: Encapsulation by spray drying has proven to be a promising technique to improve the bioavailability, release time, and mechanism of functional polyphenolic compounds as medicines, food supplements, and food additives.

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Submitted: 27 Apr 2021
Revision: 28 Aug 2021
Accepted: 28 Aug 2021
ePublished: 05 Sep 2021
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