Pharm Sci. 2021;27(2): 268-280.
doi: 10.34172/PS.2020.79
  Abstract View: 201
  PDF Download: 69

Research Article

Muscodor sp. IBRL OS-94, A Promising Endophytic Fungus of Ocimum sanctum with Antimicrobial Activity

Mohd Taufiq Mat Jalil 1,2* ORCID logo, Nabila Husna Hairudin 1,2, Darah Ibrahim 1,2

1 School of Biology, Faculty of Applied Science, Universiti Teknologi MARA, 40450, Shah Alam, Selangor, Malaysia.
2 Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.


Background: An endophytic fungus, Muscodor sp. IBRL OS-94 isolated from the leaf of Ocimum sanctum was believed to possess significant antimicrobial activity and several assays were carried out to evaluate its pharmaceutical potential.

Methods: Agar plug diffusion and the disk diffusion assays were performed to evaluate the antimicrobial activity of the fungal extract. Also, the broth microdilution assay was done to investigate the minimum inhibitory concentration (MIC) of the fungal extract. Meanwhile, the scanning electron microscope (SEM) was employed to observe the structural degeneration of the microbial cells treated to the extract.

Results: The results revealed that fungal isolate showed favorable antimicrobial activity through agar plug diffusion assay and the disk diffusion assay demonstrated that most of the test microorganisms were susceptible to extracellular extract compared to extracellular extract. As for the MIC and MLC values, the extracellular fungal extract exerted a bactericidal/fungicidal effect against all five Gram-positive bacteria, four Gram-negative bacteria, one yeast, and none of the test fungi. Meanwhile, the intracellular fungal extract exhibited bactericidal/fungicidal activity against three Gram-positive bacteria, one Gram-negative bacterium, and one yeast. The structural degeneration study via SEM revealed that various cell abnormalities including severe damage to the cell wall which led to microbial cell death.

Conclusion: The present study suggests the fungal extract from Muscodor sp. IBRLOS-94 as an antimicrobial agent.

Keywords: Antimicrobial activity, Muscodor sp. IBRL OS-94, Agar plug diffusion assay, Disk diffusion assay, MIC and MLC values, Structural degeneration
First Name
Last Name
Email Address
Security code

Abstract View: 202

Your browser does not support the canvas element.

PDF Download: 69

Your browser does not support the canvas element.

Submitted: 09 Aug 2020
Revision: 24 Sep 2020
Accepted: 28 Sep 2020
ePublished: 02 Oct 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)