Soroush Rashidpour
1, Fatemeh Zahedipour
2,3 
, Gholamreza Karimi
1,4, Khadijeh Jamialahmadi
5* 1 School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
2 Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
3 Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
4 Pharmaceutical Research Center, Institute of Pharmaceutical Technology, Mashhad University of Medical Sciences, Mashhad, Iran.
5 Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract
Background: Osthole, one of the most active components of Cnidium monnieri, has different pharmacological and biological effects such as boosting the immune system, reducing rheumatoid pain, hepatoprotective, and inhibitory effect on osteoporosis. Furthermore, it showed anti inflammatory, anti-cancer, and antioxidant properties. However, there is little information about the antioxidant effects of osthole using cell-based assays. In the current work, we used in vitro model of 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis of erythrocytes to investigate the protective effects of osthole against oxidative damage of biological membranes.
Methods: Erythrocytes were challenged with 2, 2ꞌ-azobis (2-aminopropane) dihydrochloride
(AAPH) as a model oxidant in the presence and absence of osthole. The protective effects of osthole on lipid peroxidation, protein carbonyl oxidation, glutathione (GSH) content of erythrocytes were evaluated and compared with control samples.
Results: It was found that osthole has protective effects on erythrocyte hemolysis induced by AAPH at different concentrations in a time-dependent manner. Osthole also suppressed lipid and protein oxidation as well as reductions in GSH content in a concentration and timedependent manner.
Conclusion: Osthole showed protective effects against free radical-induced hemolysis in rat
erythrocytes. Therefore, it can be considered as a supplement for the prevention or treatment
of a variety of human health problems associated with oxidative stress. However, further investigations are required to illustrate other possible impacts of osthole on cells.