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Pharm Sci. 2021;27(1): 46-55.
doi: 10.34172/PS.2020.61
  Abstract View: 239
  PDF Download: 146

Research Article

Immunomodulatory Effects of Cyperus rotundus Extract on 7,12 Dimethylbenz[a]anthracene (DMBA) Exposed BALB/c Mice

Abu Hanifah Ramadhani 1 ORCID logo, Wirdatun Nafisah 1 ORCID logo, Hary Isnanto 1 ORCID logo, Tri Kurniawati Sholeha 1 ORCID logo, Yoga Dwi Jatmiko 1 ORCID logo, Hideo Tsuboi 2, Muhaimin Rifa'i 1,3* ORCID logo

1 Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran Malang 65145, East Java, Indonesia.
2 Department of Immunology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
3 Pusat Studi Biosistem, Brawijaya University, Jl. Veteran Malang 65145, East Java, Indonesia.

Abstract

Background: The carcinogenic substance 7,12-Dimethylbenz[a]anthracene (DMBA) was commonly used to induce tumor formation in rodents. The development of tumor may trigger higher expression of pro-inflammatory cytokines, which in turn supports tumor progression.
In this study, we examined the efficacy of Cyperus rotundus extract (CRE) that was reported to have anti-inflammatory properties. We focused on investigating the levels of activated T lymphocytes and the pro-inflammatory cytokines expressed by macrophages.
Methods: Female BALB/c were injected with DMBA subcutaneously. The DMBA exposed mice were given CRE orally in three different doses; 63.33, 158.4, and 316.8 mg/kg. After 14 days, the levels of activated T lymphocytes and pro-inflammatory cytokines were analyzed using flow cytometry. Graphical analysis was done with FlowJo v10 and followed by statistical analysis.
Results: The treatment of CRE reduced the population of CD4 and CD8 T cells. The number of activated CD4 and CD8 T cells were also significantly suppressed. The population of macrophages marked by CD11b cells was significantly reduced. Finally, the CRE treatment suppressed the levels of TNF-α, IFN-γ, IL-1β, and IL-6 expressed by macrophages.
Conclusion: Our findings suggest that CRE could be a potential agent useful in therapeutic approaches for curing the disease caused by aberrant cells.
Keywords: Cyperus rotundus, DMBA, Inflammatory cytokine, Lymphocyte T cell, Macrophage
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Submitted: 06 Mar 2020
Revision: 25 Jun 2020
Accepted: 28 Jul 2020
ePublished: 02 Oct 2020
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