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Pharm Sci. 2020;26(4): 448-453.
doi: 10.34172/PS.2020.23
  Abstract View: 169
  PDF Download: 74

Short Communication

Mir-193a-5p Replacement Can Alter Metastasis Gene Expression in Breast Adenocarcinoma Cells In Vitro

Monireh Khordadmehr 1* ORCID logo, Roya Shahbazi 1 ORCID logo, Behzad Baradaran 2,3 ORCID logo, Sanam Sadreddini 2 ORCID logo, Dariush Shanehbandi 2 ORCID logo, Khalil Hajiasgharzadeh 2 ORCID logo, Masoumeh Firouzamandi 1

1 Department of Pathology, Faculty of Veterinary Medicine, University of Tabriz, 51665-1647, Tabriz, Iran.
2 Immunology Research Center, Tabriz University of Medical Sciences, 51666-14761, Tabriz, Iran.
3 Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, 51666-14761, Tabriz, Iran.

Abstract

Background: Recent evidence presented the significant role of the microRNA-193 (miR-193) family in biological processes by the contribution of specific targeting, which mainly display as a tumor suppressor in various cancers. In the present study, we evaluated the effect of miR-193a-5p replacement on some metastasis gene expression in metastatic breast cancer (BC) cells.
Methods: For this purpose, firstly, the quantitative real-time polymerase chain reaction (qRTPCR) was used to detect the miR-193a-5p expression in the MDA-MB-231 BC cell line. Subsequently, miR-193a-5p was transfected into the cells, and the expression levels of ROCK1 (Rho‑associated, coiled‑coil containing protein kinase 1), CXCR4 (Chemokine Receptor-4), CD44, and vimentin genes were evaluated by qRT-PCR.
Results: The expression level of miR-193a-5p strongly reduced in MDA-MB-231 cells. Interestingly, the ROCK1 (P < 0. 001), CD44 (P < 0.0001), CXCR4 (P < 0. 001) and vimentin (P < 0. 001) expression levels significantly decreased following miR-193a-5p transfection in MDA-MB-231 BC cells.
Conclusion: To conclude, it seems that miR-193a-5p restoration can attenuate the metastatic behavior of BC cells in vitro through decreased expression level of metastasis-related genes and may constitute an effective novel therapeutic strategy in miRNA-replacement therapy and treatment of metastatic breast adenocarcinoma in the future.
Keywords: miRNA-193, Tumor-suppressor, Breast cancer, MicroRNA replacement therapy, Metastasis genes
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Submitted: 04 Jan 2020
Revision: 29 Jan 2020
Accepted: 09 Feb 2020
ePublished: 25 Dec 2020
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