Vahid Javaherzadeh
1, Mahmood Jamshidian
1, Mohammadtaghi Zahraei
1, Arash Youseftabar
2, Morteza Milani
2, Maryam Hassan
3, Farzaneh Lotfipour
2*1 Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Gastrointestinal and Liver Disease Research Center and Faculty of Pharmacy , Tabriz University of Medical Sciences, Tabriz, Iran
3 Faculty of Pharmacy , Znjan University of Medical Sciences, Tabriz, Iran
Abstract
The objectives of the study were to isolate and
characterize the bacteriocin producing enterococci in 30 enterococcal poultry isolates from north-west
of Iran and to determine the prevalence
of bacteriocin structural genes among them. Methods: The
obtained isolates were assessed for antibacterial action against 6 indicator
strains. The PCR method was applied to detect previously identified enterocin
genes. Results: Based on our results 4 (13.3%) of the examined
poultry enterococcal isolates were considered as potential bacteriocinogenic
strains, all of them identified to be Enterococcus faecium via
phenotypic and genotypic examinations. At least one of the detected Bac+
isolates had antibacterial activities against Bacillus cereus, Listeria
monocytogenes, Enterococcus faecalis and Enterococcus hirea with no
activity against Escherichia coli or Staphylococcus aureus. Conclusion:
Genes encoding Enterocin A and
enterocin LA50A/B are fairly
distributed among the studied poultry enterococci.