Azin Jahangiri
1,2, Khosro Adibkia
2, Karim Asadpour-Zeynali
3, Yousef Javadzadeh
2, Hamed Hamishehkar
2, Mohammad Barzegar-Jalali
2*1 Students Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
2 Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
3 Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
Abstract
Background: Two
simple, precise and accurate multivariate calibration methods, partial least
square (PLS) and principal component regression (PCR) have been applied for the
simultaneous determination and dissolution profile evaluation of atorvastatin
(ATV) and ezetimibe (EZT) in their binary mixtures and commercial tablets. Due
to the closely overlapping spectral bands of the mentioned drugs, simultaneous
determination without previous separation is not possible by conventional
spectrophotometric methods. In the proposed methods (PLS and PCR) determination
of chemicals was performed by the use of a full-spectrum multivariate
calibration method.
Methods:
The experimental calibration matrix was
designed orthogonally with 16 samples composed of different mixtures of both compounds
in related mediums. The simultaneous determination of ATV and EZT was
accomplished in mixtures through recording the absorption spectra within a
range of 210 to 300 nm.
Results:
The concentration of ATV and EZT were
considered in the linear range, between 8 to 14 µg.ml-1. The
specificity of the methods was evaluated by analyzing laboratory prepared
mixtures of the mentioned drugs in specific proportions. Conclusion: The applied methods were successfully employed in
simultaneous spectrophotometric determination and dissolution profile
evaluation of ATV and EZT in their prepared mixtures and pharmaceutical
formulation.