This website might not be available on Saturday, 22 September 2019, between 08:00 IRST and 18:00 IRST, due to maintenance.
Pharm Sci. 2016;22(2):105-111.
doi: 10.15171/PS.2016.17

Scopus id: 84977106655
  Abstract View: 1439
  PDF Download: 741

Research Article

Application of Multivariate Calibration Methods, in Dissolution Testing and Simultaneous Determination of Atorvastatin and Ezetimibe in Their Combined Solid Dosage Form

Azin Jahangiri 1,2, Khosro Adibkia 2, Karim Asadpour-Zeynali 3, Yousef Javadzadeh 2, Hamed Hamishehkar 2, Mohammad Barzegar-Jalali 2 *

1 Students Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
2 Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
3 Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran

Abstract

Background: Two simple, precise and accurate multivariate calibration methods, partial least square (PLS) and principal component regression (PCR) have been applied for the simultaneous determination and dissolution profile evaluation of atorvastatin (ATV) and ezetimibe (EZT) in their binary mixtures and commercial tablets. Due to the closely overlapping spectral bands of the mentioned drugs, simultaneous determination without previous separation is not possible by conventional spectrophotometric methods. In the proposed methods (PLS and PCR) determination of chemicals was performed by the use of a full-spectrum multivariate calibration method. Methods: The experimental calibration matrix was designed orthogonally with 16 samples composed of different mixtures of both compounds in related mediums. The simultaneous determination of ATV and EZT was accomplished in mixtures through recording the absorption spectra within a range of 210 to 300 nm. Results: The concentration of ATV and EZT were considered in the linear range, between 8 to 14 µg.ml-1. The specificity of the methods was evaluated by analyzing laboratory prepared mixtures of the mentioned drugs in specific proportions. Conclusion: The applied methods were successfully employed in simultaneous spectrophotometric determination and dissolution profile evaluation of ATV and EZT in their prepared mixtures and pharmaceutical formulation.
First name
 
Last name
 
Email address
 
Comments
 
Security code


Article Viewed: 1439

Your browser does not support the canvas element.


PDF Downloaded: 741

Your browser does not support the canvas element.

Submitted: 01 Nov 2015
Accepted: 30 Nov 2015
First published online: 30 Jun 2016
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - FireFox Plugin)