Hadi Sedigh Ebrahim-Saraie
1,2, Hamid Heidari
1,2, Vahid Rezaei
1, Seyed Mohammad Javad Mortazavi
3, Mohammad Motamedifar
1,2,4*1 Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
2 Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
3 Department of Medical Physics and Medical Engineering, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
4 Shiraz HIV/AIDS Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract
Background: Recently,
nanotechnology has been demonstrated to be a promising application to overcome
the problem of antibiotic resistance. In the present study, we aimed to
determine the antibacterial activity of copper oxide nanoparticles (CuO NPs) on
several multiple-drug resistant (MDR) uropathogenic strains.
Methods:
This in vitro case-control study was performed on 4 uropathogenic bacteria including Staphylococcus
aureus, Enterococcus faecalis,
Escherichia coli and Pseudomonas aeruginosa. The antibacterial property was evaluated by
well diffusion method at different concentrations of CuO NPs.
Results:
Overall, NPs concentration of 10, 25 and 50 µg/mL showed the remarkable
antibacterial activity. A lower effect was seen against S. aureus
strains. CuO NPs exhibited maximum bacterial growth inhibition against E. faecalis strains. In most of the cases, the
zone of inhibition in 50 µg/mL concentration was closest to control positive
antibiotics.
Conclusion:
In summary, CuO NPs as an alternative to conventional
antibiotics that are currently used showed dose-dependent on antibacterial activity against different uropathogens, specificity towards pathogenic
Gram-positive bacteria. This promising antibacterial activity of CuO NPs
suggesting the development of NPs coatings on the different surface of biomedical materials for applications in
different antimicrobial control systems.