Niusha Sharifi
1, Shabnam Mahernia
2, Massoud Amanlou
1,2*1 Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
2 Pharmaceutical Science Research Center, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Background: Raphanus sativus (Radish) is a plant of the Brassicaceae
family which is grown worldwide. This plant has been used in Iranian
traditional medicine as a laxative, abortifacient and also recently as
anti-tumor, anti-proliferative and anti-diabetic agent.
Quercetin, the most important flavonoid which is found
in this plant, serves as an antioxidant and as a result an anticancer agent.
Methods: The present study was designed to compare extraction
efficiency of different methods with various solvents in quercetin extraction
from R. sativus leaves. The analysis of extracts was performed
using high performance liquid chromatography (HPLC) and the quantification was
carried out by the use of quercetin calibration curve.
Results: In consequence, Ultrasonic Assisted Extraction (UAE)
method at 50% of ultrasound intensity (Frequency of 50/60 KHz) for 10 min in
methanol proved to be the most efficient technique in quercetin extracting
(11.8% yield).
Conclusions: This study demonstrated the optimized condition for
quercetin extraction from R. sativus leaves, which is due to promoting
larger agitation (cavitation) in UAE method.