Nidal Amin Jaradat
1*, Motasem Al-Masri
2, Fatmeh Hussen
1, Abed Naser Zaid
1, Iyad Ali
3, Ala Tammam
1, Doaa Mostafa Odeh
1, Omaima Hussein Shakarneh
1, Areej Rajabi
11 Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine.
2 Department of Biology and Biotechnology, Faculty of Science, An-Najah National University, Nablus, Palestine.
3 Department of Biochemistry and Genetics, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, Palestine.
Abstract
Background: Since ancient times, the treatment of
various diseases has relied on medicinal
plants. The replacement of chemically
synthesized drugs with natural medications gave the later advantageous due to
their safety for humans.
This study aimed to screen and to evaluate
preliminarily phytoconstituents, total
contents of flavonoids, tannins, and phenols also to evaluate the antibacterial
and antioxidant activities of Cyclamen coum
aerial parts methanol extract.
Methods:
Screening of phytoconstituents, total
flavonoids, tannins and phenols contents, as well as antioxidant properties, were investigated for the Cyclamen
coum aerial parts by using standard phytochemical and
analytical methods. Evaluation of antibacterial activity of the plant
methanolic extract was performed by using broth microdilution method. American Type Culture
Collections of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus (MRSA) were
utilized to evaluate antibacterial activity.
Results: Preliminary phytochemical qualitative analysis of the
plant methanolic extract showed that the plant aerial parts contained amino
acids, reducing sugar, carbohydrate, tannin, flavonoid, phenol, saponin,
cardiac glycoside, and steroids. Total
flavonoids, phenols, and tannins in plant
methanolic extract were 60.88 mg QUE/g, 32.7
mg GAE/g and 11.7 mg CAE/g, respectively. Meanwhile, the antioxidant activity
was estimated to be 31 µg/mL and has very weak antibacterial activity on the
studied pathogens.
Conclusion:
In the
present study, evaluation of total phenolic, tannins and flavonoid content from
the aerial parts of C. coum can be an antioxidant naturally
potent source. The results of in-vitro antibacterial studies
showed that the studied plant has very weak antibacterial activity.
In addition, the presence of antioxidant compounds in C. coum makes it a good
candidate for manufacturing dietary supplements and food preservatives.