Pharm Sci. 2015;21(4):205-210.
doi: 10.15171/PS.2015.38

Scopus id: 84954559666
  Abstract View: 769
  PDF Download: 523

Research Article

Phytochemical Analysis, Quantitative Estimations of Total Phenols and Free Radical Scavenging Activity of Bupleurum subovatum from Jerusalem

Nidal Jaradat 1 * , Ahmad Eid 1, Fatima Abdelwahab 1, Linda Isa 1, Amjad Abdulrahman 1, Murad Abualhasan 1, Azmi Mahmoud Ali Hussein 2

1 Department of Pharmacy, Faculty of Medicine and Health Sciences, An - Najah National University, Nablus, Palestine
2 Department of Biomedical Sciences, Faculty of Medicine, An - Najah National University , Nablus, Palestine

Abstract

The crude extracts from Bupleurum subovatum plant were used to screen the presence of secondary metabolic products, to estimate the total phenol content and free radical scavenging activity for the plant extracts. Methods:Antioxidant activity was evaluated by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay, while total phenol content was determined by using Folin Ciocalteu' s method. Result:The phytochemical analysis confirmed the presence of phenol, proteins, starch, reducing sugars, tannins, volatile oils, cardiac glycosides, steroid, and huge amounts of saponins. Total phenolic content in the methanolic extract was 9.05 mg/g Gallic acid. At the same time, methanolic extract showed a mild potential oxygen free radical scavenging ability as well as the IC50 for the plant was 18.60 ± 0.36 µg/ml, which justified its uses in the folkloric medicine and could be further subjected for the isolation of their therapeutic active compounds. Conclusion:The results of this study revealed the antioxidant activity and confirmed the therapeutic usage of Bupleurum subovatum in the traditional medicine.
First name
 
Last name
 
Email address
 
Comments
 
Security code


Article Viewed: 769

Your browser does not support the canvas element.


PDF Downloaded: 523

Your browser does not support the canvas element.

Submitted: 29 Jul 2015
Accepted: 16 Sep 2015
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - FireFox Plugin)