Nidal Jaradat
1*, Ahmad Eid
1, Fatima Abdelwahab
1, Linda Isa
1, Amjad Abdulrahman
1, Murad Abualhasan
1, Azmi Mahmoud Ali Hussein
21 Department of Pharmacy, Faculty of Medicine and Health Sciences, An - Najah National University, Nablus, Palestine
2 Department of Biomedical Sciences, Faculty of Medicine, An - Najah National University , Nablus, Palestine
Abstract
The
crude extracts from Bupleurum subovatum plant were used to screen the
presence of secondary metabolic products, to estimate the total phenol content
and free radical scavenging activity for the plant extracts. Methods:Antioxidant activity was
evaluated by using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay, while
total phenol content was determined by using Folin Ciocalteu' s method. Result:The
phytochemical analysis
confirmed the presence of phenol,
proteins, starch, reducing sugars, tannins, volatile oils, cardiac glycosides,
steroid, and huge amounts of saponins. Total phenolic content in the methanolic extract was
9.05 mg/g Gallic acid. At the same time, methanolic
extract showed a mild potential oxygen free radical scavenging ability as well as the IC50 for the plant was 18.60 ±
0.36 µg/ml, which justified its uses in the folkloric medicine and could be further subjected for the isolation of
their therapeutic active compounds. Conclusion:The results of
this study revealed the antioxidant activity and confirmed the therapeutic
usage of Bupleurum subovatum in the traditional medicine.