Reza Mohebbati
1, Mohammad Jalili-Nik
2, Maryam Paseban
1, Mohammad Naser Shafei
1, Abolfazl Khajavirad Rad
3*1 Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
2 Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
3 Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract
Background: Due to the antioxidant
effects of Zataria multiflora (ZM) and Carvacrol (CAR) in various
problems and the prominent role of the ROS in neurotoxicity induced by
Doxorubicin (DOX), this study was designed to investigate the effects of ZM
hydroalcoholic extract and CAR on DOX-induced oxidative stress in rat brain
Methods: 24 male rats were randomly divided into four groups including: 1)Control
,2)Doxorubicin (DOX) that received DOX via a tail vein on the first day of the
study, 3,4) ZM+DOX and CAR+DOX which received ZM and CAR by gavage for 28
consecutive days. Brain tissue removed
for redox markers evaluation.
Results: MDA
level in the DOX group was significantly increased compared to control group
while in treated groups did not show any significant changes in comparison with
the DOX group. Also, Thiol content in DOX group showed significant reduction
compared to control group. Thiol contents in treated groups showed no
significant difference compared to DOX group. Catalase (CAT) activity, an
antioxidant enzyme, in the DOX group were significantly decreased compared to
control group and increased in treated rats in comparison with the DOX group.
Activity of Superoxide dismutase (SOD), an antioxidant enzyme, in the DOX group
was significantly reduced compared to control group and increased in treated
rats in comparison with the DOX group.
Conclusion: The
present study showed that ZM hydroalcoholic extract and CAR could inhibit DOX
induced oxidative stress of the brain mainly with effect on the enzymatic
antioxidant defense system.