Mortoza Rashtbar, Daryoush Mohammadnejad, Ali Abed Elahi, Amaneh Mohammadi Roushandeh*
Abstract
One of the main factors which could have side effects in spermatogenesis is
used in chemotherapy to treat cancer patients. It is proved that the agents
affect on cells especially with high proliferation such as spermatocytes and
kill them through apoptosis induction. Since gonadotropin hormones (FSH, LH)
affect spermatogenesis and are suppressed by chemotherapy agents, so
administration of GnRH Antagonist to protect the cells against its side
effects is in researchers’ interest. Present study tried to investigate
protective effects of cetrorelix in suppression of side effects of cisplatin as
chemotherapy agent on the germinal epithelium. Methods: In the
present study 30 male mice were divided randomly into 3 groups: Control,
Experimental 1 received 2.5 mg/kg cisplatin daily intraperitonealy and
Expermental 2 received cisplatin and subcutaneously injection of 0.25 mg/kg
cetrorelix. Cetrorelix injection initiated 1 week before cisplatin treatment
and continued at second and third weeks. After 35 days of last injection, the
testes were removed and processed for histological and apoptotic analysis
according to routine protocols. Results: Histological findings of
seminiferous tubules showed that in cisplatin receiving group, number of
spermatogonia cells, thickness of germinal epithelium, seminiferous tubules
diameter and spermatogenesis index rate (SI) were decreased. Also in some of
the tubules only sertoli cells were observed. In addition, our study showed
that number of apoptotic cells was increased in this group. Cetrorelix had
prohibitive potential against side effects of cisplatin via morphology of
seminiferous tubules and decline apoptotic cells. Conclusion:
Cisplatin has adverse side effects on germinal epithelium by induction
apoptosis and cetrorelix can protect spermatogenesis process from cisplatin
adverse side effects.