Fatemeh Fathiazad
1, Maryam Rameshrad
2, Samira Asghari
3, Sanaz Hamedeyazdan
1, Alireza Garjani
2, Nasrin Maleki-Dizaji
2*1 Department of Pharmacognosy, Tabriz University of Medical Sciences, Tabriz, Iran
2 Department of Pharmacology and Toxicology, Tabriz University of Medical Sciences, Tabriz, Iran
3 Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract
Background: The upward desire in
using traditional medicine as a remedy for treatment of different diseases has
led the scientists to be thoughtful on plants as alternative sources of
conventional drugs.
Methods: Herein,
anti-inflammatory effects of Marrubium vulgare methanolic extract was
evaluated in carrageenan- induced paw edema in rats through examining paw
thickness, histological studies and myeloperoxidase activity (MPO). The
antioxidant activity of M. vulgare extract and its phenolic and
flavonoids content were evaluated by folin-Ciocalteau, and aluminum chloride colorimetric assay, separately.
Results: The results showed
that M. vulgare alleviated paw inflammation as indexed by reduction paw
thickness (p<0.001) as well as MPO activity (p<0.001), which was
associated with a marked decrease in tissue edema. Moreover, the extract RC50
value for DPPH and nitric oxide antioxidant activity was 177 µg/mL and 370.5
µg/ml, besides, the total phenolic and flavonoids were as 625 mg gallic acid
equivalent and 1.62 g quercetin equivalent per 100 g of dried plant material.
Conclusion: In the main, the
observed anti-inflammatory and antioxidant properties of M. vulgare
could be attributed to the high amounts of phenolic and flavonoid content
identified in the extract.