Pharm Sci. 2015;20(4): 149-156.
  Abstract View: 723
  PDF Download: 645

Original Research

The effects of Ocimum basilicum ethanol extract on carrageenan induced paw inflammation in rats 

Maryam Rameshrad 1,2, Ronak Salehian 2, Fatemeh Fathiazad 3, Sanaz Hamedeyazdan 3, Mehraveh Garjani 2, Nasrin Maleki-Dizaji 1 * , Reza Vosooghi 2

1 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
2 Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
3 Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran


Background: Traditionally, Ocimum basilicum has been used for its anti-inflammatory activity. Here, the effect of O. basilicum ethanol extract was evaluated on carrageenan induced paw edema in male Wistar rats. Methods: O. basilicum ethanol extract was achieved through maceration procedure. Next, the paw inflammation was induced with 1% carrageenan, subcutaneous, to all groups after 1h of the intrapritoneal injection of O. basillicum extracts (0, 2.5, 5 and 10mg/kg; respectively). The paw thickness was measured at hourly intervals for 4h. Finally, histological examination and Myeloperoxidase (MPO) activity was assessed in the inflamed paw at the 4thh. Moreover, total phenolic and flavonoid content of the extract was determined. Besides, different in vitro antioxidant activities were detected by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, nitric oxide radical inhibition and reducing power methods. Results: The extract diminished paw inflammation as indexed by reduction paw thickness (p<0.001) as well as MPO activity (p<0.001), which was associated with a marked decrease in tissue edema and leukocyte infiltration. In addition, values for the total phenolic and flavonoids were calculated as 266 mg gallic acid equivalent and 65 mg quercetin equivalent per 100g of O. basilicum fresh plant material. Furthermore, the RC50 values for DPPH and nitric oxide antioxidant activity of the extract was determined as 118µg/mL and 929.7µg/ml. Conclusions: Ethanol extract of O. basilicum significantly decreased the inflammatory reaction induced by carrageenan which could be related to its antioxidant effects.
Keywords: O. basilicum, antioxidant, carrageenan, MPO, paw inflammation
First Name
Last Name
Email Address
Security code

Abstract View: 723

Your browser does not support the canvas element.

PDF Download: 645

Your browser does not support the canvas element.

Submitted: 29 Dec 2014
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)