Haleh Vaez
1, Alireza Garjani
1, Abbas Delazar
2,3, Maryam Rameshrad
4, Negisa Seyed Toutounchi
5, Elhameh Nikkhah
2, Parina Asgharian
2,3*1 Department of Pharmacology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
5 Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
Background: Coronary artery disease is a leading cause of
death worldwide. The present study has been designed to investigate efficacy of
methanolic extract of Scrophularia subuphylla (S. subuphylla)
on ischemia and reperfusion-induced myocardial injury in isolated rat heart.
Methods: The
isolated male Wistar rat hearts (n= 5) were perfused by Krebs-Henseleit
solution enriched with the extract (0, 1, 5, and 10 μg/ml), using the
langendorff method. After 15 minutes stabilization, the hearts were subjected
to 30 minutes regional ischemia and then 120 minutes reperfusion.
Results: Administration
of the extract did not improve any of cardiac markers of flow
rate, heart rate and developed pressure. Number, percentage and duration
of arrhythmias were not affected by any concentrations
of the extract. However, the concentration of 1 and 5 µg/ml increased the VT
duration compared to control group (P<0.05). Furthermore,
there was not considerable infarct size difference between none of the groups.
Methanolic extract did not show any cardioprotective effect, while it had high
anti-oxidant activity as well as high amounts of total phenol and flavonoid
contents.
Conclusion: Generally, the methanolic extract of S. subuphylla at the doses which studied exhibited no protective effects
against I/R-induced injures, which might be due to the high amount of cardiac
glycosides with low therapeutic index.