Pharm Sci. 2017;23(3):193-197.
doi: 10.15171/PS.2017.29

Scopus id: 85026748083
  Abstract View: 404
  PDF Download: 654

Research Article

Effects of Analytical Procedures on the Repeatability of Malondialdehyde Determinations in Biological Samples

Sajad Azizi 1, Maryam Khoubnasabjafari 2 * , Aziz Shahrisa 3, Mehry Khoubnasabjafari 4, Jafar Soleymani 5, Abolghasem Jouyban 1,6

1 Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
4 Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
5 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
6 Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

Background: Malondialdehyde (MDA) is a commonly used biomarker of oxidative stress in clinical studies and has been measured in many pathological conditions during last decades. Different analytical methods have been reported for determination of MDA in biological samples in which MDA was adducted with thiobarbituric acid (TBA) to produce more sensitive chromophore and also convert it to a fluorescent compound. In spite of the routine applications of this derivatization and subsequent analysis of MDA in biomedical studies, its reliability, repeatability and reproducibility is questionable. The aim of this work is to investigate the effects of some factors on the repeatability of MDA determinations in standard solutions and also in plasma samples using spectroscopic method. Methods: MDA-TBA adduct is prepared in standard solutions and the effects of pH, temperature, reaction time, open, closed and reflux systems and the ratio of MDA and TBA is investigated by measuring the absorbance of the solution at 532 nm. These effects are also investigated in human plasma samples. Results: The best results are obtained at pH 2.5, temperature of 70 °C, reaction time of 150 minutes, reflux system and ratio of 2. Conclusion: Using the optimized conditions are resulted in better repeatability.
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Submitted: 10 Nov 2016
Accepted: 26 Jan 2017
First published online: 30 Sep 2017
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