Snehal Salunke
1, Vishal Pande
2*, Prakash Kendre
2, Somnath Vibhute
21 Department of Pharmaceutics and Quality Assurance, Sanjivani College of Pharmaceutical Education and Research, Kopargaon, Dist. Ahmadnagar , India
2 Department of Pharmaceutics (PG), Sanjivani College of Pharmaceutical Education and Research, Kopargaon 423603 , India
Abstract
Standardization
of polyherbal formulation is mandatory to evaluate the best quality of drugs
for therapeutic value. The main objective of this study was to develop a
standardized, safe and effective polyherbal juice with significant anti-oxidant
and anti-diabetic activity. Methods: Polyherbal juice was
formulated using cold maceration method and was evaluated for Total phenolic
content, Total flavonoid content, Antioxidant activity, Particle size, IR,
HPTLC and Heavy metal analysis. Results: Total phenolic content
was found to be 139.23 ± 0.072
mg/g gallic acid equivalent and
Total flavonoid content was 62.01± 0.063 mg/g Catechin equivalent. In DPPH scavenging assay
the RC50 value of polyherbal formulation was found to be 6.0 µg/ml. Preliminary
phytochemical tests and HPTLC was performed for Quantitative Analysis of
Phytoconstituents in formulation. Conclusion: In-vivo study shows
significant anti-diabetic activity as compared with marketed formulations. Thus
polyherbal formulation possesses good anti-diabetic and antioxidant activity.