Abstract
Background: Several mechanism
including autoxidation of glucose, protein glycation and advanced glycation end
products (AGEs) formation may to be involved in hyperglycemia mediated
oxidative stress. Herein, in this study, the effects of rutin with high antioxidant
activity isolated from Teucrium
polium on
structural changes of human serum albumin (HSA) were performed. Methods:
The major compounds
of T. polium methanol
extract were isolated and their radical scavenging properties were established
through various approaches including
1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radicals scavenging, and
reducing power. The structural changes of HSA with glucose, in the presence
of rutin were studied with far-UV CD spectroscopy. Results: Based on our
results, rutin, a flavonol glycoside isolated from T. polium exhibited
high antioxidant activity compared to the other isolated compounds from T.
polium. Rutin inhibited high glucose induced oxidative damages to protein
by decreasing protein carbonyl
formation (PCO) and preventing thiols group from oxidation. In addition,
evaluating the structural changes of HSA by glucose in the presence of rutin
showed that rutin may be able to increase the helicity of the protein and
prevent helix decrement in the secondary structure of HSA. Conclusion:
We found that rutin with high antioxidant activity effectively inhibited the
generation of amadori
products and exhibited good inhibitory effects on the formations of
α-dicarbonyl compounds and AGEs.