Abstract
Background: The OCT4B1 as a new discovered variant of OCT4 is expressed in
both cancer cell and tissues. This variant with its anti-apoptotic properties
aid cancer cells to scape from apoptosis. TNF ligands and receptors are amongst
two categories of eleven gene families involved in the apoptosis pathway.
Therefore, the aim of the present study was to investigate the effects of
OCT4B1 suppression on several transcripts of both TNF ligands and receptors
family in some tumor cell lines. Methods: The AGS (gastric
adenocarcinoma), 5637 (bladder tumor) and U-87MG (brain tumor) tumor cell lines
were transfected with specific OCT4B1 siRNA and , as well as a scrambled
sequence and PBS, as controls, using Lipofectamine 2000 comerical kit. The
expression of TNF ligand and receptor transcripts were evaluated in parallel
with beta-actin (as housekeeping gene) using Real-Time PCR technique. Results:
our results indicated that in TNF ligand transcripts family, the mRNA level of
TNF transcripts was up-regulated and inversely TNFSF8, TNFSF7, TNFSF10, TNFSF1
and TNFSF6 was down-regulated. We observed also that in TNF receptor
transcripts family, six transcripts including, TNFRSF 10A, TNFRSF10B,
TNFRSF11B, TNFRSF1A, TNFRSF21 and TNFRSF25 were up-regulated, while TNFRSF9 and
CD27 were down-regulated. Conclusions: According to these
results, it may be concluded that OCT4B1 suppression can lead to apoptosis in
tumor cell lines via up-regulation of several TNF ligand and receptor
transcripts. Thus, OCT4B1 suppression effects on TNF and its receptors may be
considered as promising target genes in future studies in cancr research and
therapy.