Pharm Sci. 2018;24(4):298-303.
doi: 10.15171/PS.2018.43

Scopus id: 85060060860
  Abstract View: 97
  PDF Download: 123

Research Article

Development of a Micellar Electrokinetic Chromatographic Method with Indirect UV Detection for Pregabalin Determination in Serum Samples 

Hamid Reza Sargazi 1, Elnaz Tamizi 2, Elaheh Rahimpour 3, Abolghasem Jouyban 4,5 * ORCiD

1 Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran.
2 Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Pharmaceutical Analysis Research Center, Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran.
5 Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

Background: A micellar electrokinetic chromatographic (MEKC)/ indirect UV detection method with hydrodynamic and electrokinetic injection has been developed for the determination of pregabalin in the serum samples. Methods: Separation of the drug was achieved on Agilent capillary electrophorese in less than 5 min using a 50 cm × 75 μm i.d. uncoated fused-silica capillary and a background electrolyte (BGE) consisting of 5-aminosalicylic acid (5-ASA, 10 mmol L-1), cetyl trimethylammonium bromide (CTAB, 1 mmol L-1) and tri-sodium citrate (4% w/v). The influence of various parameters on the separation such as separation voltage, injection time, cassette temperature, pH of BGE and organic modifier was investigated. Results: Method validation shown good linearity (R2> 0.999) in the range of 1.5-100 µg mL-1 of pregabalin. A limit of detection (LOD) of 0.8 μg mL-1 and a limit of quantitation (LOQ) of 2.6 μg mL-1 were reported for pregabalin. Conclusion: The proposed method was found to be suitable and accurate for the determination of pregabalin in serum samples.
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Submitted: 08 Jun 2018
Revised: 23 Jun 2018
Accepted: 05 Jul 2018
First published online: 30 Dec 2018
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