Hamid Reza Sargazi
1, Elnaz Tamizi
2, Elaheh Rahimpour
3, Abolghasem Jouyban
4,5* 
1 Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran.
2 Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Pharmaceutical Analysis Research Center, Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Iran.
5 Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
Background: A micellar electrokinetic
chromatographic (MEKC)/ indirect UV detection method with hydrodynamic and
electrokinetic injection has been developed for the determination of pregabalin
in the serum samples.
Methods: Separation of the drug was
achieved on Agilent capillary
electrophorese in less than 5 min using a 50 cm × 75 μm i.d. uncoated fused-silica
capillary and a background electrolyte (BGE) consisting of 5-aminosalicylic
acid (5-ASA, 10 mmol L-1), cetyl trimethylammonium bromide (CTAB, 1
mmol L-1) and tri-sodium citrate (4% w/v). The influence of various
parameters on the separation such as separation voltage, injection time,
cassette temperature, pH of BGE and organic modifier was investigated.
Results: Method validation shown good linearity (R2>
0.999) in the range of 1.5-100 µg mL-1 of pregabalin. A limit of detection (LOD) of 0.8 μg mL-1
and a limit of quantitation (LOQ) of 2.6 μg mL-1 were reported for pregabalin.
Conclusion: The
proposed method was found to be suitable and accurate for the determination of pregabalin in
serum samples.