Farzad Farajbakhsh
1,2, Mohammad Amjadi
2, Jamshid Manzoori
3, Mohammad R. Ardalan
4, Abolghasem Jouyban
5,6*1 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 Department of Analytical Chemistry, Faculty of Chemistry, Tabriz University, Tabriz, Iran
3 Department of Chemistry, Tabriz Islamic Azad University, Tabriz, Iran
4 Chronic Kidney Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5 Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
6 Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract
Background: Analysis of aluminium (Al) in urine
samples is required in management of a number of diseases including patients
with renal failure. This work aimed to present dispersive liquid-liquid
microextraction (DLLME) and ultrasound-assisted
emulsification microextraction (USAEME) methods for the preconcentration of ultra-trace
amount of aluminum in human urine prior to its determination by a graphite
furnace atomic absorption spectrometry (GFAAS).
Methods:
The microextraction methods were based on the complex formation of Al3+
with 8-hydroxyquinoline. The effect of various experimental parameters on the
efficiencies of the methods and their optimum values were studied.
Results:
Under the optimal conditions, the limits of detection for USAEME-GFAAS and
DLLME-GFAAS were 0.19 and 0.30 ng mL−1, respectively and corresponding
relative standard deviations (RSD, n=5) for the determination of 40 ng mL−1
Al3+ were 5.9% and 4.9%.
Conclusion:
Both methods could be successfully used to the analysis of ultra trace
concentrations of Al in urine samples of dialysis patients.