Zahra Azarifar
1, Motahareh Mortazavi Mortazavi
2, Rozbeh Farhadian
2, Soraya Parvari
3, Amaneh Mohammadi roushandeh
2*1 Faculty of Science, Payamenoor University of Tehran, Tehran, Iran
2 Anatomical Sciences Department, Medicine Faculty, Hamadan University of Medical Sciences, Hamadan, Iran
3 Anatomical Sciences Department, Medicine Faculty, Alborz University of Medical Sciences, Karaj, Iran
Abstract
Background:
Progressive incidence of cervical cancer persuaded the
scientists to find the most effective and appropriate treatment approaches such
as herbal medicine. Hence, it was aimed in this study to evaluate antitumoral
effects of Purtulaca
oleracea (P. oleracea) aqueous extract on HeLa cell line. Materials:
HeLa cell line was cultured and submitted to different concentrations of P.
oleracea extract for 24 and 48 hours. Cell proliferation and viability were
conducted by MTT assay and trypan blue staining respectively. Results:
our results showed that cell proliferation was decreased significantly along
with increase in P. oleracea extract concentrations as well as time
lapse (p < 0.05). Also, the same result was obtained for cell viability. Conclusion:
it is suggested that P. oleracea aqueous extract has antiprolifrative
effects on HeLa cell line depending on concentration and time. Though, it
appears that this extract decreased the growth of HeLa cells but its
cytotoxicity on non-tumoral cells must be considered. Also, it is necessary to
know which mechanisms cause its antiprolifrative properties.