Tabriz University of Medical Sciences Pharmaceutical Sciences 1735-403X 32 2 2026 04 30 Investigation of Nose-to-Brain Transportation of Peptide-Displaying Bacteriophage Particles Using Phage Display Method 302 308 10.34172/PS.026.43481 EN Hakimeh Kanaani https://orcid.org/0000-0001-5411-815X Nasibeh Mohammad Ali Pourradi Navid Kaboudi Siavoush Dastmalchi Yadollah Azarmi https://orcid.org/0000-0002-0016-0034 Maryam Hamzeh-Mivehroud https://orcid.org/0000-0002-1257-0102 Journal Article 10.34172/PS.026.43481 2025 11 11 2025 12 29 Background: Brain drug delivery is of paramount significance in CNS-related diseases as blood brain barrier (BBB) permeability is not feasible for many pharmaceutical agents. The purpose of this study was to identify peptide sequences with specific translocation property into brain via intranasal route using in vivo phage display method to be used as CNS drug delivery carriers. Methods: To screen peptides with nose-to-brain translocation capability, a 12-mer peptide library displayed on M13 bacteriophage was intranasally administered to anesthetized mice with subsequent recovery of the phage particles from the brain. The identified peptide sequences were analyzed using bioinformatics tools. Results: The results showed that intranasal transport of phage particles to the brain is independence of displayed peptide sequences due to random distribution of residues in different positions of isolated peptides. Nanoscale feature of bacteriophage particles may be responsible for nose-to-brain translocation through olfactory epithelium. Conclusion: Taken together, the results open a route for designing phage-guided therapeutic systems as nanocarriers useful in intranasal brain targeting drug delivery.