Tabriz University of Medical Sciences Pharmaceutical Sciences 1735-403X 26 4 2020 12 25 Mir-193a-5p Replacement Can Alter Metastasis Gene Expression in Breast Adenocarcinoma Cells In Vitro 448 453 10.34172/PS.2020.23 EN Monireh Khordadmehr https://orcid.org/0000-0002-4472-3847 Roya Shahbazi https://orcid.org/0000-0001-7728-4919 Behzad Baradaran https://orcid.org/0000-0002-8642-6795 Sanam Sadreddini https://orcid.org/0000-0002-4996-1297 Dariush Shanehbandi https://orcid.org/0000-0002-9449-0607 Khalil Hajiasgharzadeh Masoumeh Firouzamandi Journal Article 10.34172/PS.2020.23 2020 01 04 2020 02 09 Background: Recent evidence presented the significant role of the microRNA-193 (miR-193) family in biological processes by the contribution of specific targeting, which mainly display as a tumor suppressor in various cancers. In the present study, we evaluated the effect of miR-193a-5p replacement on some metastasis gene expression in metastatic breast cancer (BC) cells. Methods: For this purpose, firstly, the quantitative real-time polymerase chain reaction (qRTPCR) was used to detect the miR-193a-5p expression in the MDA-MB-231 BC cell line. Subsequently, miR-193a-5p was transfected into the cells, and the expression levels of ROCK1 (Rho‑associated, coiled‑coil containing protein kinase 1), CXCR4 (Chemokine Receptor-4), CD44, and vimentin genes were evaluated by qRT-PCR. Results: The expression level of miR-193a-5p strongly reduced in MDA-MB-231 cells. Interestingly, the ROCK1 (P < 0. 001), CD44 (P < 0.0001), CXCR4 (P < 0. 001) and vimentin (P < 0. 001) expression levels significantly decreased following miR-193a-5p transfection in MDA-MB-231 BC cells. Conclusion: To conclude, it seems that miR-193a-5p restoration can attenuate the metastatic behavior of BC cells in vitro through decreased expression level of metastasis-related genes and may constitute an effective novel therapeutic strategy in miRNA-replacement therapy and treatment of metastatic breast adenocarcinoma in the future.