Logo-ps
Pharm Sci. 2022;28(3): 481-491.
doi: 10.34172/PS.2021.69

Scopus ID: 85139869129
  Abstract View: 529
  PDF Download: 314

Research Article

Potential Anthelmintic and Antioxidant Activities of Jasminum fruticans L. and Its Phytochemical Analysis

Esra Küpeli Akkol 1* ORCID logo, Esma Kozan 2 ORCID logo, Hilal Bardakci 3 ORCID logo, Timur Hakan Barak 3 ORCID logo, Sara Khalilpour 1

1 Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330, Ankara, Turkey.
2 Deparment of Parasitology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey.
3 Department of Pharmacognosy, Faculty of Pharmacy, Acibadem Mehmet Ali Aydınlar University, 34752, Istanbul, Turkey.
*Corresponding Author: Corresponding Author: Esra Küpeli Akkol, E-mail: , Email: esrak@gazi.edu.tr

Abstract

Background: Ethnobotanical investigations conducted in Turkey demonstrated that Jasminum fruticans L. extract and fruit juice had been used against parasites in animals. In this study, the possible antihelmintic activity of various J. fruticans extracts contributing to its traditional use, was relatively assessed. In addition, the antioxidant potentials and phytochemical composition of the extracts were investigated since there is a relationship between helminthiasis, oxidative stress and phenolic metabolites.

Methods: In this study, aerial parts of J. fruticans were subsequently extracted using n-hexane, ethyl acetate (EtOAc) and methanol (MeOH). In vivo anthelmintic activity of the extracts was compared with albendazole used as a reference in adult earthworms. Various methods, including free radical scavenging and metal-related activity assays, were used to assess the antioxidant capacity of the above-mentioned extracts. Assessment of phenolic composition was accomplished through total phenolic, phenolic acid, and flavonoid content assays as well as liquid chromatography-mass spectrometry (LC-MS/MS) using multiple reaction monitoring (MRM) scan modes. Further chlorogenic acid (3-O-caffeoylquinic acid) contents of extracts were quantified using high-performance thin-layer chromatography (HPTLC).

Results: Between all tested extracts, MeOH extract at a quantity of 50.0 mg/mL, paralysed worms in 8.1 min and killed them in 12.8 min, showing a high anthelmintic effect similar to albendazole. Similarly, in vitro DPPH radical scavenging activity, cupric ion reduction and total antioxidant capacity experiments demonstrated that MeOH extract had significant antioxidant activity. Further phytochemical screening showed that MeOH extract was richer regarding phenolic metabolites. Chlorogenic acid, ferulic acid, caffeic acid and gallic acid were only detected in the MeOH extract.

Conclusion: Results justify and support the use of J. fruticans in traditional medicine as an anthelmintic agent. Furthermore, a positive correlation was found between the strong antioxidant capacity along with the phenolic composition determined in the MeOH extract and anthelmintic activity.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 530

Your browser does not support the canvas element.


PDF Download: 314

Your browser does not support the canvas element.

Submitted: 09 Jun 2021
Revision: 15 Nov 2021
Accepted: 16 Nov 2021
ePublished: 23 Nov 2021
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)