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Pharm Sci. 2020;26(4): 379-385.
doi: 10.34172/PS.2020.47
  Abstract View: 226
  PDF Download: 138

Research Article

The Combination Effect of Voluntary Exercise and Crocin on Angiogenic miRNAs in High-Fat Diet/Low-Dose STZ-Induced Type2 Diabetes in Rats: miR-126 and miR-210

Hassan Dariushnejad 1,2 ORCID logo, Leila Chodari 3,4, Vajihe Ghorbanzadeh 2* ORCID logo

1 Department of Biotechnology, Lorestan University of Medical Sciences, Khorramabad, Iran.
2 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
3 Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran.
4 Department of Medical Physiology, Urmia University of Medical Sciences, Urmia, Iran.

Abstract

Background: As one of the major complications of diabetes, cardiovascular disease might result in early death in people with diabetes. miR-126 and 210 expressions undergo alterations in cardiac disease and cause heart failure.
Methods: Animals were divided into the 5 groups of control (Con), diabetes (Dia), diabeticcrocin (Dia-Cro), diabetic-voluntary exercise (Dia-Exe), and diabetic-crocin-voluntary exercise (Dia-Cro-Exe). Type 2 diabetes was induced by the use of a high-fat diet (4 weeks) and injection of streptozotocin (STZ) (i.p, 35 mg/kg). Animals received crocin orally (50 mg/kg), and voluntary exercise was performed alone or together for 8 weeks. QRT–PCR method was used to determine the levels of miR-210 and miR-126 in cardiac tissue.
Results: The levels of miR-210 and miR-126 in the cardiac tissue augmented in both the crocin and voluntary exercise groups in comparison with the non-treated group (p<0.001). The use of combination therapy with exercise and crocin magnified their effects on miR-210 and miR-126 levels (p<0.001). Moreover, MiR-210 levels were lower in the crocin group compared to the exercise group (p<0.001).
Conclusion: The results indicated that voluntary exercise combined with crocin might provide a novel therapeutic plan for cardiovascular disease through increasing miR-210 and miR-126 expression.
Keywords: miR-126, miR-210, Type 2 diabetes, Crocin, Voluntary exercise
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Submitted: 30 Nov 2019
Revision: 30 May 2020
Accepted: 30 May 2020
ePublished: 25 Dec 2020
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