Logo-ps
Pharm Sci. 2020;26(2): 209-213.
doi: 10.34172/PS.2020.6
  Abstract View: 105
  PDF Download: 58

Short Communication

Antityrosinase Inhibitory Activity of Phytochemicals from Alpinia aquatica Roscoe

Nissha Bharrathi Romes 1 ORCID logo, Wan Mohd Nuzul Hakimi Wan Salleh 2 * ORCID logo, Hasnah Mohd Sirat 1 * ORCID logo, Zaini Assim 3 ORCID logo

1 Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 Johor Bahru, Johor, Malaysia.
2 Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris (UPSI), 35900 Tanjong Malim, Perak, Malaysia.
3 Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Kota Samarahan, 93400 Kuching, Sarawak, Malaysia.

Abstract

Background: Genus Alpinia are commonly used as spices and ingredients in traditional medicines. In the present study, we attempted to isolate the phytochemicals from Alpinia aquatica and evaluate their tyrosinase inhibitory activity.
Methods: Phytochemical constituents of the extract were investigated using various chromatographic and spectroscopic methods. The chemical structures of the isolated phytochemicals were established by analysis of their spectroscopic data, as compared to that of reported data. Tyrosinase inhibitory activity was also tested on the extracts and selected compounds using mushroom tyrosinase as the enzyme.
Results: Fractionation and purification of the extracts of Alpinia aquatica afforded seven known compounds which are 5-hydroxy-3,7,4’-trimethoxyflavone (1), 4’,5-dihydroxy-3,7-dimethoxyflavone (2), 2-methoxy-8-(2’,4’,5’-trimethoxyphenyl)-1,4-naphthaquinone (3), cis-3S-(2’,4’,5’-trimethoxyphenyl)-4S-[(E)-2’’’,4’’’,5’’’-trimethoxystyryl]cyclohexene (4), 2,4,5-trimethoxybenzaldehyde (5), stigmasterol (6) and β-sitosterol (7). The ethyl acetate extract of pseudostems possessed the highest tyrosinase inhibition of 31.0% among the extracts, while compound (1) gave tyrosinase inhibition of 48.0%.
Conclusion: Compounds (3) and (4) were isolated for the first time from A. aquatica and Alpinia genus. These phytochemical results suggest that the extracts could assist as a potential source of bioactive compounds. Further research is needed in which the extract could possibly be exploited for pharmaceutical use.
Keywords: Zingiberaceae, Alpinia aquatica, Tyrosinase, Flavonoid, Phytochemical
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 105

Your browser does not support the canvas element.


PDF Download: 58

Your browser does not support the canvas element.

Submitted: 15 Nov 2019
Accepted: 29 Dec 2019
ePublished: 27 Jun 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)