Pharm Sci. 2020;26(2): 209-213.
doi: 10.34172/PS.2020.6

Scopus ID: 85089456433
  Abstract View: 413
  PDF Download: 271

Short Communication

Antityrosinase Inhibitory Activity of Phytochemicals from Alpinia aquatica Roscoe

Nissha Bharrathi Romes 1 ORCID logo, Wan Mohd Nuzul Hakimi Wan Salleh 2* ORCID logo, Hasnah Mohd Sirat 1* ORCID logo, Zaini Assim 3 ORCID logo

1 Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia (UTM), 81310 Johor Bahru, Johor, Malaysia.
2 Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris (UPSI), 35900 Tanjong Malim, Perak, Malaysia.
3 Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Kota Samarahan, 93400 Kuching, Sarawak, Malaysia.


Background: Genus Alpinia are commonly used as spices and ingredients in traditional medicines. In the present study, we attempted to isolate the phytochemicals from Alpinia aquatica and evaluate their tyrosinase inhibitory activity.
Methods: Phytochemical constituents of the extract were investigated using various chromatographic and spectroscopic methods. The chemical structures of the isolated phytochemicals were established by analysis of their spectroscopic data, as compared to that of reported data. Tyrosinase inhibitory activity was also tested on the extracts and selected compounds using mushroom tyrosinase as the enzyme.
Results: Fractionation and purification of the extracts of Alpinia aquatica afforded seven known compounds which are 5-hydroxy-3,7,4’-trimethoxyflavone (1), 4’,5-dihydroxy-3,7-dimethoxyflavone (2), 2-methoxy-8-(2’,4’,5’-trimethoxyphenyl)-1,4-naphthaquinone (3), cis-3S-(2’,4’,5’-trimethoxyphenyl)-4S-[(E)-2’’’,4’’’,5’’’-trimethoxystyryl]cyclohexene (4), 2,4,5-trimethoxybenzaldehyde (5), stigmasterol (6) and β-sitosterol (7). The ethyl acetate extract of pseudostems possessed the highest tyrosinase inhibition of 31.0% among the extracts, while compound (1) gave tyrosinase inhibition of 48.0%.
Conclusion: Compounds (3) and (4) were isolated for the first time from A. aquatica and Alpinia genus. These phytochemical results suggest that the extracts could assist as a potential source of bioactive compounds. Further research is needed in which the extract could possibly be exploited for pharmaceutical use.
Keywords: Zingiberaceae, Alpinia aquatica, Tyrosinase, Flavonoid, Phytochemical
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Submitted: 15 Nov 2019
Accepted: 29 Dec 2019
ePublished: 27 Jun 2020
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