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Pharm Sci. 2019;25(4): 345-351.
doi: 10.15171/PS.2019.43

Scopus ID: 85078851707
  Abstract View: 163
  PDF Download: 141

Research Article

Simultaneous Determination of Phenobarbital, Phenytoin, Carbamazepine and Carbamazepine-10,11-epoxide in Plasma of Epileptic Patients

Azam Samadi 1 ORCID logo, Maryam Khoubnasabjafari 2 * ORCID logo, Mohammad Barzegar 3, Shahram Sadeghvand 4, Shadi Shiva 5, Abolghasem Jouyban 1,6 ORCID logo

1 Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Science, Tabriz, Iran.
3 Pediatric Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Student Research Committee, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
5 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
6 Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

Background: Quantitative analyses of antiepileptic drugs are required in clinic and to rational dosage adjustment, the clinician needs the blood levels of these drugs. A high-performance liquid chromatography with spectrophotometric detection has been developed and validated for simultaneous determination of some antiepileptic drugs in plasma of patients with epilepsy. Methods: A simple procedure based on deproteinization by acetonitrile was used for pre-treatment of plasma samples. Liquid chromatographic analysis was carried out on a Nova-Pak® C18 analytical column, using a ternary mixture of potassium dihydrogen phosphate buffer (pH 6.0)-acetonitrile-2-propanol (63:22:15, v/v/v) as the mobile phase, at a flow rate of 1.0 mL min-1. Results: Calibration curves were linear over a range of 1–40 µg mL-1 for phenobarbital, 1–30 µg mL-1 for phenytoin, 0.3–15 µg mL-1 for carbamazepine and 0.5–6 µg mL-1 for carbamazepine epoxide. Conclusion: The simple sample pre-treatment, combined with the fast chromatographic run was used for the determination of antiepileptic drugs for a large number of patient samples.
Keywords: Phenobarbital, Carbamazepine, Carbamazepine metabolite, Phenytoin, HPLC analysis, Therapeutic drug monitoring
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Submitted: 12 Apr 2019
Revision: 08 May 2019
Accepted: 27 May 2019
ePublished: 20 Dec 2019
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