Sara Shojaei Zarghani
1, Hamid Soraya
2, Leila Zarei
3, Mohammad Alizadeh
4*1 Student Research Committee, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
2 Cellular and Molecular Research Center, Department of Pharmacology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran
3 Solid tumor research center, Urmia University of Medical Sciences, Urmia, Iran
4 Food and Beverages Safety Research Center, Department of Nutrition, Faculty of Medicine, Urmia University of Medical Sciences , Urmia, Iran
Abstract
Background:
There are many
methods for inducing non-alcoholic fatty liver disease (NAFLD) in experimental
animals. Due to the diversity of these methods and different variables involved
in choosing the appropriate one, this study aimed to examine the effect of
three different diets on development of NAFLD in rats.
Methods: Twelve rats
were divided to receive a standard, high fat high fructose (HFHFr), high
cholesterol high fructose (HCHFr) or high fat high sucrose diet (HFHS); with
access to tap water, fructose or sucrose solutions. The liver histopathological
and biochemical assessments were examined after 40 and 60 days.
Results: According
to the histological findings, after 60 days of dietary exposures, all three
experimental groups showed evidence of fatty changes; however a higher grade of
ballooning and NAFLD activity score was found in the HFHFr compared with the
other groups. Furthermore, all three diets induced a non-significant increase
in serum liver enzymes relative to the control diet.
Conclusion: This study
indicates that HFHFr diet induce higher grade of hepatic steatosis and
ballooning degenerations after 60 days in comparison with the other groups. So
HFHFr diet can be considered as a suitable method for inducing of fatty liver
for nutritional and pharmacological studies.