Pharm Sci. 2019;25(1):65-69.
doi: 10.15171/PS.2019.10
  Abstract View: 103
  PDF Download: 116

Research Article

Chromatographic Method for Determination of the Amino Acid Content in Dioscorea bulbifera L. Tubers by RP-HPLC

Musadiq Hussain Bhat 1 * ORCiD, Mufida Fayaz 1, Amit Kumar 2, Alamgir Ahmad Dar 3, Ashok Kumar Jain 2

1 School of Studies in Botany, Jiwaji University, Gwalior-474011, India.
2 Institute of Ethnobiology, Jiwaji University, Gwalior-474011, India.
3 Research Centre for Residue and Quality Analysis, Sher‐e‐Kashmir University of Agricultural Sciences and Technology (SKUAST‐K), Srinagar, J&K- 180009, India.

Abstract

Background: The present study was carried out for determination of amino acid content in tubers of Dioscorea bulbifera using reverse-phase high-performance liquid chromatography. Methods: The method involved the vapor phase hydrolysis of the sample, automated derivatisation of the amino acids with the aid of AccQ-Fluor reagent kit, separated on a high performance liquid chromatography equipped with photo diode array (HPLC-PDA) at 254 nm having column temperature of 37 ºC. Results: The proportional molar concentration for each amino acid was calculated based on the concentration of standard amino acids and expressed as μg amino acid/mg sample. Methionine, aspartic acid and leucine were major components while as tyrosine was found minor from the plant on dry weight basis. Conclusion: The method is reliable, simple and economical for determining the amino acid content of Dioscorea bulbifera tubers.

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Submitted: 26 Jul 2018
Revised: 03 Nov 2018
Accepted: 25 Nov 2018
First published online: 18 Mar 2019
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