Parina Asgharian
1,2, Abbas Delazar
1,2, Farzaneh Lotfipour
2, Solmaz Asnaashari
3*1 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
Background: The current study was assigned to evaluate the
antioxidant, general toxicity, anti-proliferative and antimicrobial activities
of different extracts obtained from rhizomes of Eremostachys macrophylla
(Lamiaceae).
Methods: All activities were evaluated by obtaining extracts
of E. macrophylla in n-hexane, DCM (dichloromethane) and MeOH (methanol)
by soxhlet apparatus. The antioxidant activity of the extracts was evaluated in
terms of FRST (free radical scavenging activity test) by DPPH (2,
2-diphenyl-1-picrylhydrazyl). BSLT (Brine shrimp lethality tests), MTT (3-[4,
5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay and disc
diffusion method were carried out to determine the general toxicity,
anti-proliferative and antibacterial activities of the different extracts,
respectively.
Results: The findings
of the study for antioxidant, anti-proliferative and antibacterial effects
showed that DCM extract was the most active fraction, but n-hexane extract
indicated the most potent effect against Artemia salina.
Conclusion: The results revealed strong bioactive effects of
nonpolar fractions of E. macrophylla rhizomes. Thus, it is possible to
suggest some new potential antioxidant, cytotoxic and antibacterial agents with
no harmful effects on noncancerous cells.