Abstract
Background:
Vitamin E is a
fat–soluble agent protecting cells from free radicals damages. Previous studies
have shown that oxidative stress plays an important role in mucosal intestinal
damages in burn trauma. This study aimed
to investigate vitamin E effects on small intestinal mucosal changes in burned
rats. Methods: Mature male rats (n=32) weighing 260 ± 10 g
were used in this experiment. After induction of deep general anesthesia, a
determined area of rats’ back region (10% of body surface) was exposed to 95oC
water for 8 seconds to induce a second-degree wet burn. The evaluated groups in
our study were: 8 rats without burning, 8 rats without burning treated with
vitamin E, 300 mg/kg/day for 15 days, 8 burned rats without medication and 8
burned rats treated with vitamin E, 300 mg/kg/day for 15 days. All rats were
killed on fifteenth day by ether inhalation. The samples were taken from the
first part of small intestine and were stained by Hematoxylin & Eosin
method. Results: Burned
rats receiving vitamin E had a higher intestinal villi height and lower
intestinal lumen diameter as compared to burned rats without the vitamin E
treatment (P < 0.05 and P < 0.01, respectively) and those values were
close to the results of unburned ones. There were no significant differences
among the study groups regarding the intestinal diameter and muscular layer
thickness. Conclusion:
Vitamin E can improve intestinal villus height and lumen diameter and its
consumption at the time of burning may protect intestine mucosa.