Akbar Rezazadeh
1,2, Mohammad Amjadi
3, Jamshid L Manzoori
4, Alireza Ghaffari
5, Abolghasem Jouyban
1,6*1 Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Research & Development, Shahid Ghazi Pharmaceutical Company, Tabriz, Iran.
3 Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
4 Department of Chemistry, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
5 Medical Philosophy and History Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
6 Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz, Iran.
Abstract
Background: A new, fast and sensitive spectrofluorimetric
method was proposed for the determination of furosemide in serum samples based
on a dispersive liquid-liquid microextraction.
Methods: The optimum
conditions for quantification of furosemide were studied considering the
effects of types and amounts of dispersive and extraction solvents, salt
addition, pH value, rate and duration of centrifugation. The method was
validated with respect to the linearity, recovery and limit of detection.
Results: Under the optimal
conditions, the fluorescence intensities at 406 nm (with the excitation
wavelength of 342 nm) were linear with the concentration of furosemide in the
range of 0.3 to 20 µg mL-1, with a detection
limit of 0.12 µg mL-1 and a relative standard deviation of 3.4–9.4%.
Conclusion: Careful examination
of the obtained validation results reveal that the proposed method is suitable
for determination of furosemide in serum samples.