Pharm Sci. 2017;23(4):264-270.
doi: 10.15171/PS.2017.39

Scopus id: 85040196514
  Abstract View: 435
  PDF Download: 910

Research Article

LC-MS/MS Estimation of Propranolol level in Exhaled Breath Condensate

Samin Hamidi 1, Maryam Amini 2, Maryam Khoubnasabjafari 3, Vahid Jouyban-Gharamaleki 4,5, Hossein Sate 6, Abolghasem Jouyban 5,7 *

1 Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
2 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4 Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
5 Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Science, Tabriz, Iran.
6 Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
7 Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

Background: Exhaled breath condensate (EBC) could be used as a non-invasive and alternative specimen to urine and blood for monitoring propranolol levels. A simple, sensitive and selective liquid chromatography–tandem mass spectrometry (LC–MS/MS) method is employed for the determination of propranolol in EBC samples. Methods: Samples directly injected to a C18 analytical column and isocratically separated using a mobile phase composed of methanol + acetic acid (99:1 v/v). Detection was performed by positive electrospray ionization in multiple reaction monitoring and selected ion recording modes. Results: The chromatographic separation was obtained within 6.0 min and was linear over the concentration range of 5.6–224.0 ng/mL (R2 = 0.999). The accuracy and precision of the method were within 15% according to FDA guideline. The found concentrations of propranolol in EBC of two patients receiving 80 mg/day were 30 and 40 ng/mL. Conclusion: Developed method was applied to determine propranolol levels in three patients receiving propranolol in their medication. The obtained propranolol levels in EBC could be used to develop simpler, cheaper and more feasible analytical methods to be used in routine analysis of propranolol in biomedical analytical laboratories.
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Submitted: 06 Apr 2017
Accepted: 24 Aug 2017
First published online: 30 Dec 2017
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